gal 4 based yeast hybrid system Search Results


α yeast gal4 polyclonal  (Santa Cruz Biotechnology)
95
Santa Cruz Biotechnology α yeast gal4 polyclonal
α Yeast Gal4 Polyclonal, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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α yeast gal4 polyclonal - by Bioz Stars, 2026-09
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gal4 transcription factor activation- (ad-) domain  (Becton Dickinson)
90
Becton Dickinson gal4 transcription factor activation- (ad-) domain
Gal4 Transcription Factor Activation (Ad ) Domain, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gal+4+based+yeast+hybrid+system/gal4+dna+binding+domain/pmc04070083-173-10-15
Average 90 stars, based on 1 article reviews
gal4 transcription factor activation- (ad-) domain - by Bioz Stars, 2026-09
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plasmid pbind (encoding the yeast gal4 dna binding domain upstream of a multiple cloning region, mcr)  (Promega)
90
Promega plasmid pbind (encoding the yeast gal4 dna binding domain upstream of a multiple cloning region, mcr)
Plasmid Pbind (Encoding The Yeast Gal4 Dna Binding Domain Upstream Of A Multiple Cloning Region, Mcr), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gal+4+based+yeast+hybrid+system/gal4+responsive+luciferase+reporter/pmc06605256-387-5-52
Average 90 stars, based on 1 article reviews
plasmid pbind (encoding the yeast gal4 dna binding domain upstream of a multiple cloning region, mcr) - by Bioz Stars, 2026-09
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yeast gal4 dna  (Addgene inc)
93
Addgene inc yeast gal4 dna
( A ) Schematic representation of the plasmid system used to measure PTC suppression in BRCA1 . Human BRCA1 (residues 1396–1863) containing an in-frame UAG PTC was expressed as a genetic fusion to the <t>Gal4</t> DNA-binding domain (Gal4DBD). Three copies of tRNA V2.1 were expressed from the same plasmid. The Gal4DBD-BRCA1 plasmid was co-transfected with a plasmid containing the firefly luciferase (Fluc) gene and five copies of the Gal4 upstream activation sequence (5× UAS). In this assay, Fluc transcription is dependent on BRCA1-mediated transactivation. ( B ) BRCA1 activity in HEK 293 cells expressing BRCA1 with a UAG PTC at the indicated position with (+) and without (–) co-expression of tRNA V2.1 . Data are displayed as the mean ± SEM of three biological replicates. * P < 0.05, ** P < 0.005, unpaired student’s t -test.
Yeast Gal4 Dna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gal+4+based+yeast+hybrid+system/pRS415-RSC2+(Plasmid+%2311001)/pmc11662663-199-2-19
Average 93 stars, based on 1 article reviews
yeast gal4 dna - by Bioz Stars, 2026-09
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yeast gal4 rna  (ATCC)
99
ATCC yeast gal4 rna
( A ) Schematic representation of the plasmid system used to measure PTC suppression in BRCA1 . Human BRCA1 (residues 1396–1863) containing an in-frame UAG PTC was expressed as a genetic fusion to the <t>Gal4</t> DNA-binding domain (Gal4DBD). Three copies of tRNA V2.1 were expressed from the same plasmid. The Gal4DBD-BRCA1 plasmid was co-transfected with a plasmid containing the firefly luciferase (Fluc) gene and five copies of the Gal4 upstream activation sequence (5× UAS). In this assay, Fluc transcription is dependent on BRCA1-mediated transactivation. ( B ) BRCA1 activity in HEK 293 cells expressing BRCA1 with a UAG PTC at the indicated position with (+) and without (–) co-expression of tRNA V2.1 . Data are displayed as the mean ± SEM of three biological replicates. * P < 0.05, ** P < 0.005, unpaired student’s t -test.
Yeast Gal4 Rna, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gal+4+based+yeast+hybrid+system/Yeast/us07759066-244-12-20
Average 99 stars, based on 1 article reviews
yeast gal4 rna - by Bioz Stars, 2026-09
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maxi kit qiagen 12362 matchmaker goldtm gal4 based yeast  (Qiagen)
99
Qiagen maxi kit qiagen 12362 matchmaker goldtm gal4 based yeast
( A ) Schematic representation of the plasmid system used to measure PTC suppression in BRCA1 . Human BRCA1 (residues 1396–1863) containing an in-frame UAG PTC was expressed as a genetic fusion to the <t>Gal4</t> DNA-binding domain (Gal4DBD). Three copies of tRNA V2.1 were expressed from the same plasmid. The Gal4DBD-BRCA1 plasmid was co-transfected with a plasmid containing the firefly luciferase (Fluc) gene and five copies of the Gal4 upstream activation sequence (5× UAS). In this assay, Fluc transcription is dependent on BRCA1-mediated transactivation. ( B ) BRCA1 activity in HEK 293 cells expressing BRCA1 with a UAG PTC at the indicated position with (+) and without (–) co-expression of tRNA V2.1 . Data are displayed as the mean ± SEM of three biological replicates. * P < 0.05, ** P < 0.005, unpaired student’s t -test.
Maxi Kit Qiagen 12362 Matchmaker Goldtm Gal4 Based Yeast, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gal+4+based+yeast+hybrid+system/EndoFree+Plasmid+Maxi+Kit/pm31091452-200-117-119
Average 99 stars, based on 1 article reviews
maxi kit qiagen 12362 matchmaker goldtm gal4 based yeast - by Bioz Stars, 2026-09
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anti gal4 dna binding domain monoclonal antibody  (Santa Cruz Biotechnology)
95
Santa Cruz Biotechnology anti gal4 dna binding domain monoclonal antibody
( A ) Schematic representation of the plasmid system used to measure PTC suppression in BRCA1 . Human BRCA1 (residues 1396–1863) containing an in-frame UAG PTC was expressed as a genetic fusion to the <t>Gal4</t> DNA-binding domain (Gal4DBD). Three copies of tRNA V2.1 were expressed from the same plasmid. The Gal4DBD-BRCA1 plasmid was co-transfected with a plasmid containing the firefly luciferase (Fluc) gene and five copies of the Gal4 upstream activation sequence (5× UAS). In this assay, Fluc transcription is dependent on BRCA1-mediated transactivation. ( B ) BRCA1 activity in HEK 293 cells expressing BRCA1 with a UAG PTC at the indicated position with (+) and without (–) co-expression of tRNA V2.1 . Data are displayed as the mean ± SEM of three biological replicates. * P < 0.05, ** P < 0.005, unpaired student’s t -test.
Anti Gal4 Dna Binding Domain Monoclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gal+4+based+yeast+hybrid+system/GAL4+Antibody/pmc00085938-97-16-22
Average 95 stars, based on 1 article reviews
anti gal4 dna binding domain monoclonal antibody - by Bioz Stars, 2026-09
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yeast strain y190  (ATCC)
91
ATCC yeast strain y190
( A ) Schematic representation of the plasmid system used to measure PTC suppression in BRCA1 . Human BRCA1 (residues 1396–1863) containing an in-frame UAG PTC was expressed as a genetic fusion to the <t>Gal4</t> DNA-binding domain (Gal4DBD). Three copies of tRNA V2.1 were expressed from the same plasmid. The Gal4DBD-BRCA1 plasmid was co-transfected with a plasmid containing the firefly luciferase (Fluc) gene and five copies of the Gal4 upstream activation sequence (5× UAS). In this assay, Fluc transcription is dependent on BRCA1-mediated transactivation. ( B ) BRCA1 activity in HEK 293 cells expressing BRCA1 with a UAG PTC at the indicated position with (+) and without (–) co-expression of tRNA V2.1 . Data are displayed as the mean ± SEM of three biological replicates. * P < 0.05, ** P < 0.005, unpaired student’s t -test.
Yeast Strain Y190, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gal+4+based+yeast+hybrid+system/Y-190/us07601739-187-1-20
Average 91 stars, based on 1 article reviews
yeast strain y190 - by Bioz Stars, 2026-09
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plasmid gal4 vp16  (Addgene inc)
93
Addgene inc plasmid gal4 vp16
( A ) Schematic representation of the plasmid system used to measure PTC suppression in BRCA1 . Human BRCA1 (residues 1396–1863) containing an in-frame UAG PTC was expressed as a genetic fusion to the <t>Gal4</t> DNA-binding domain (Gal4DBD). Three copies of tRNA V2.1 were expressed from the same plasmid. The Gal4DBD-BRCA1 plasmid was co-transfected with a plasmid containing the firefly luciferase (Fluc) gene and five copies of the Gal4 upstream activation sequence (5× UAS). In this assay, Fluc transcription is dependent on BRCA1-mediated transactivation. ( B ) BRCA1 activity in HEK 293 cells expressing BRCA1 with a UAG PTC at the indicated position with (+) and without (–) co-expression of tRNA V2.1 . Data are displayed as the mean ± SEM of three biological replicates. * P < 0.05, ** P < 0.005, unpaired student’s t -test.
Plasmid Gal4 Vp16, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gal+4+based+yeast+hybrid+system/Gal4-VP16+(Plasmid+%2371728)/pmc12204705-128-14-21
Average 93 stars, based on 1 article reviews
plasmid gal4 vp16 - by Bioz Stars, 2026-09
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gal 4 dna  (Vector Laboratories)
96
Vector Laboratories gal 4 dna
( A ) Schematic representation of the plasmid system used to measure PTC suppression in BRCA1 . Human BRCA1 (residues 1396–1863) containing an in-frame UAG PTC was expressed as a genetic fusion to the <t>Gal4</t> DNA-binding domain (Gal4DBD). Three copies of tRNA V2.1 were expressed from the same plasmid. The Gal4DBD-BRCA1 plasmid was co-transfected with a plasmid containing the firefly luciferase (Fluc) gene and five copies of the Gal4 upstream activation sequence (5× UAS). In this assay, Fluc transcription is dependent on BRCA1-mediated transactivation. ( B ) BRCA1 activity in HEK 293 cells expressing BRCA1 with a UAG PTC at the indicated position with (+) and without (–) co-expression of tRNA V2.1 . Data are displayed as the mean ± SEM of three biological replicates. * P < 0.05, ** P < 0.005, unpaired student’s t -test.
Gal 4 Dna, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gal+4+based+yeast+hybrid+system/galactose/10__1074_slash_jbc__m601502200-62-19-22
Average 96 stars, based on 1 article reviews
gal 4 dna - by Bioz Stars, 2026-09
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pbind-gr  (Promega)
90
Promega pbind-gr
( A ) Schematic representation of the plasmid system used to measure PTC suppression in BRCA1 . Human BRCA1 (residues 1396–1863) containing an in-frame UAG PTC was expressed as a genetic fusion to the <t>Gal4</t> DNA-binding domain (Gal4DBD). Three copies of tRNA V2.1 were expressed from the same plasmid. The Gal4DBD-BRCA1 plasmid was co-transfected with a plasmid containing the firefly luciferase (Fluc) gene and five copies of the Gal4 upstream activation sequence (5× UAS). In this assay, Fluc transcription is dependent on BRCA1-mediated transactivation. ( B ) BRCA1 activity in HEK 293 cells expressing BRCA1 with a UAG PTC at the indicated position with (+) and without (–) co-expression of tRNA V2.1 . Data are displayed as the mean ± SEM of three biological replicates. * P < 0.05, ** P < 0.005, unpaired student’s t -test.
Pbind Gr, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gal+4+based+yeast+hybrid+system/pbind+gr/us10711032-2381-22-28
Average 90 stars, based on 1 article reviews
pbind-gr - by Bioz Stars, 2026-09
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gal4 dna binding domain  (Thermo Fisher)
99
Thermo Fisher gal4 dna binding domain
( A ) Schematic representation of the plasmid system used to measure PTC suppression in BRCA1 . Human BRCA1 (residues 1396–1863) containing an in-frame UAG PTC was expressed as a genetic fusion to the <t>Gal4</t> DNA-binding domain (Gal4DBD). Three copies of tRNA V2.1 were expressed from the same plasmid. The Gal4DBD-BRCA1 plasmid was co-transfected with a plasmid containing the firefly luciferase (Fluc) gene and five copies of the Gal4 upstream activation sequence (5× UAS). In this assay, Fluc transcription is dependent on BRCA1-mediated transactivation. ( B ) BRCA1 activity in HEK 293 cells expressing BRCA1 with a UAG PTC at the indicated position with (+) and without (–) co-expression of tRNA V2.1 . Data are displayed as the mean ± SEM of three biological replicates. * P < 0.05, ** P < 0.005, unpaired student’s t -test.
Gal4 Dna Binding Domain, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gal+4+based+yeast+hybrid+system/DNA/pmc03877814-404-46-54
Average 99 stars, based on 1 article reviews
gal4 dna binding domain - by Bioz Stars, 2026-09
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Image Search Results


( A ) Schematic representation of the plasmid system used to measure PTC suppression in BRCA1 . Human BRCA1 (residues 1396–1863) containing an in-frame UAG PTC was expressed as a genetic fusion to the Gal4 DNA-binding domain (Gal4DBD). Three copies of tRNA V2.1 were expressed from the same plasmid. The Gal4DBD-BRCA1 plasmid was co-transfected with a plasmid containing the firefly luciferase (Fluc) gene and five copies of the Gal4 upstream activation sequence (5× UAS). In this assay, Fluc transcription is dependent on BRCA1-mediated transactivation. ( B ) BRCA1 activity in HEK 293 cells expressing BRCA1 with a UAG PTC at the indicated position with (+) and without (–) co-expression of tRNA V2.1 . Data are displayed as the mean ± SEM of three biological replicates. * P < 0.05, ** P < 0.005, unpaired student’s t -test.

Journal: Nucleic Acids Research

Article Title: Efficient suppression of premature termination codons with alanine by engineered chimeric pyrrolysine tRNAs

doi: 10.1093/nar/gkae1048

Figure Lengend Snippet: ( A ) Schematic representation of the plasmid system used to measure PTC suppression in BRCA1 . Human BRCA1 (residues 1396–1863) containing an in-frame UAG PTC was expressed as a genetic fusion to the Gal4 DNA-binding domain (Gal4DBD). Three copies of tRNA V2.1 were expressed from the same plasmid. The Gal4DBD-BRCA1 plasmid was co-transfected with a plasmid containing the firefly luciferase (Fluc) gene and five copies of the Gal4 upstream activation sequence (5× UAS). In this assay, Fluc transcription is dependent on BRCA1-mediated transactivation. ( B ) BRCA1 activity in HEK 293 cells expressing BRCA1 with a UAG PTC at the indicated position with (+) and without (–) co-expression of tRNA V2.1 . Data are displayed as the mean ± SEM of three biological replicates. * P < 0.05, ** P < 0.005, unpaired student’s t -test.

Article Snippet: Briefly, the yeast Gal4 DNA-binding domain (Gal4; residues 1–100) was cloned from plasmid Gal4-VP16 (a gift from Lea Sistonen; Addgene plasmid # 71728; http//n2t.net/addgene:71728 ; RRID:Addgene_71728) as a genetic fusion to human BRCA1 (residues 1396–1863) (a gift from Stephen Elledge; Addgene plasmid #14999; http://n2t.net/addgene:14999 ; RRID:Addgene_14999) in a pCDNA3.1 plasmid harboring three copies of the gene encoding tRNA V2.1 under control of a 7SK promoter.

Techniques: Plasmid Preparation, Binding Assay, Transfection, Luciferase, Activation Assay, Sequencing, Activity Assay, Expressing